This platform allows electronic data management and is compatible with. General algorithm mgit 960 cultures 43 flow chart 2. Software driven result interpretation sire susceptibility test features. Polymerase chain reaction pcr was performed with the advansure tuberculosis tbnontuberculosis mycobacterium ntm realtime pcr kit lg life sciences, seoul, korea. Bactec mgit 960 system is an automated system for the growth and detection of mycobacteria with a capacity to incubate and continuously monitor 960 mycobacteria growth indicator tube mgit every 60 minutes for increase in fluorescence rohner et al. The reliability of the bactec mgit 960 system, an automated version of the. Studies indicate that mgit 960 has a tendency to indicate more inh resistance rates.
Pdf failure of bactec mgit 960 to detect mycobacterium. Numerous studies have been carried out using mgit system for primary isolation of mycobacteria as compared with lj and ogawa. For more information on how the bactec mgit 960 system and the new mgit tube for paratb will provide these benefits to your johnes lab, please contact bd at 877. The keyword all returns both gnd and ast test results that are currently in the instrument. Reliability of mycobacterial growth indicator tube mgit. Comparison of recoveries of mycobacterium tuberculosis. Bactec mgit 960 system principle, manual and brochure. Bbl mgit mycobacteria growth indicator tube, oadc enrichment, panta antibiotic mixture bbl mgit mycobacteria growth indicator tube 7 ml with bactec mgit 960 supplement kit bbl mycoprep specimen digestiondecontamination kit for processing of. Evaluation of bactec mycobacteria growth indicator tube mgit. Comparison study of genexpert versus tb mgit culture in.
Comparison of the recovery of mycobacterium bovis isolates. The optional bd epicenter data management system performs statistical. Mgit early positive cultures znbap negative 46 11 solid culture. Direct and indirect pza susceptibility testing was performed as described previously by siddiqi et al. Safe and efficient onboard incubation eliminates sharps, and the need to handle or transfer tubes once the system is loaded, reducing labor requirements while. Bbl mgit system is the manual system while bactec mgit 960 mgit 960 is the fully automatic system for detection of mycobacterial growth and drug susceptibility testing of m. Mycobacteria growth indicator tube mgit is intended for the detection and recovery of mycobacteria. Time needed for test completion was found to be almost similar for these methods. Quantitative drug susceptibility testing of mycobacterium. Bd bactec mgit automated mycobacterial detection system bd. The bd bactec mgit 960 system is for highvolume mycobacteria growth, detection and susceptibility testing and is designed to meet the needs of medium and highvolume laboratories. We assessed the performance of rema in comparison with bactec mgit 960 in the susceptibility testing of 80 mycobacterium tuberculosis clinical isolates from clemente ferreira institute against four drugs. The bd bactec mgit 960 system is for highvolume mycobacteria growth.
A total of 120 mycobacterial isolates were recovered from 1,068 clinical specimens. Evaluation of bactec mgit 960 system for testing susceptibility of. Bd life sciences diagnostic systems package inserts bd. The mean detection time of mycobacteria on bactec mgit 960. The bd bactec mgit 960 instrument is a fully automated system for the rapid. From 1985 to 1992, the number of reported cases of tuberculosis increased 18%. The bactec 12b media isolated significantly more m. Evaluation of the versatrek system compared to the bactec.
Fourteen samples had overgrowth in bactec mgit 960 tubes but grew mycobacteria with at least one other culture medium. For the direct method, sputum specimens were processed by using the nacetyllcysteinesodium hydroxide nalcnaoh method, using a final concentration of 1 to 1. Addendum and paratb application software bactec mgit. Its performance was compared to those of the radiometric bactec 460 instrument and eggbased lowensteinjensen. Comparison of the mgit 960, bactec 460 tb and solid media. Lj solid culture and mycobacteria growth indicator tube mgit liquid culture systems and culturebased correlates for bacillary density from the sputum of hivtbcoinfected individuals at baseline were. Learn about bd bactec mgit susceptibility testing reagents. Bd bactec mgit tubes only require the addition of the bd bactec mgit 960 supplement prior to specimen inoculation and incubation. The bactec 460 tb system has been replaced with the new nonradiometric bactec mgit mycobacterial growth indicator tube 960 system 9, 15, 20, designed for the detection of mycobacterial species commonly found in human clinical samples 5, 15, 17. Data management, bd epicenter data management system. Mycotb is an inexpensive method to simultaneously identify the minimal inhibitory concentrations mics of twelve first and secondline drugs.
Bactec mgit 960 system are well comparable to those generated by the radiometric bactec 460tb system. Evaluation of the bactec mgit 960 and the mbbact systems for. The mean detection time of mycobacteria on bactec mgit 960 system was 11. There are a few published reports on the evaluation of bactec mgit 960 on extrapulmonary samples. Bd leads in health care safety including medication safety, health care worker safety.
Use of bactec mgit 960 for recovery of mycobacteria from. Bactec mgit 960 system principle, manual and brochure the bactec mgit 960 mycobacterial detection system is the worlds first automated system for highvolume mycobacteria growth, detection and susceptibility testing from becton dickinson bd. The mgit 960 system has several advantages over the widely used bactec 460 system. Use of mgit 960 in routine mycobacteriology has dramatically reduced the turn around time for detection of resistance thus paving way for early and accurate intervention. Rifampin rmp, isoniazid inh, streptomycin sm, and ethambutol emb were tested at the following concentrations. Class 2 device recall bd bactectm mgit tm 960 system. The software is compatible with the hospitals existing microsoft windows computer network, providing simultaneous access to all connected staff. Susceptibility testing of mycobacterium tuberculosis to. We have evaluated the bactec 460 tb against mgit 960, and a good correlation was observed between the two methods for all the drugs tested in this model. However, contamination rates were much higher for the mgit media, 1724%, compared to 7% for bactec, suggesting that contamination was a major cause of mgit. If any does not, block the stations as described in section 6. Bd bbl mycoprep specimen digestiondecontamination kit for processing mycobacterial specimens. Bactec mgit paratb instrument 445870 bactec mgit paratb starter kit 440979 includes mgit 960 paratb users manual addendum and paratb application software bactec mgit paratb medium tube 245154 pkg of 100 bactec mgit paratb supplement 245156 pkg of 6 x 15 ml for more information on how the bactec mgit 960 system. The mgit mycobacteria growth indicator tube contains 7 ml of modified middlebrook 7h9 broth base.
Evaluation of bactec mgit 960 system for recovery of. The aim of this study was to evaluate the reliability of the versatrek system for mycobacterium tuberculosis drug susceptibility testing compared with results obtained with the bactec mgit 960 system. Comparison of resazurin microtiter assay performance and. The bactec tm mgit tm system software is used in the mgit 960 and mgit 320 instruments for the rapid detection of mycobacteria from clinical specimens. Learn about bd life sciences diagnostic systems package inserts. Bd epicenter delivers healthcare professionals with a library of readytouse epidemiology reports designed to discover and monitor hai outbreaks and trends.
Test status id q, 5, 1, 2 the status of the specimens being. Evaluation of the bactec mgit 960 and the mbbact systems. This software provides advanced data management for all bd microbiology systems. Though requiring more technical time, the manual system is a lot more affordable for laboratories with a smaller budget.
Services, bd bactec mgit 960 or 320 instrument service, includes service and replacement parts for years two and three. Mycobacteria growth indicator tube, oadc enrichment, bd panta antibiotic mixture. Comparison of ogawa media, bactec mgit 960 system and. From january to december 2009, 635 specimens were cultured with mycobacteria growth indicator tube mgit and ogawa media. Rema proved to be a rapid and accurate method, providing excellent correlation with bactec mgit 960, with the exception of results for the ethambutol drug. Password reset return labels crystal id registration. Bactec mgit software intended application culture medium tubes. Pdf comparison of the conventional culture, the manual. Correlation between the bactec mgit 960 culture system. Concerning time to result there is no statistically significant difference between the two systems 7. A comparison of the sensititre mycotb plate, the bactec. A fully automated bactec mgit 960 system a mgit was compared with conventional media and the mycobacteria growth indicator tube m mgit method by shitaye et al. Bd bactec mgit mycobacteria growth indicator tubes bd. Automation of culture using the bactec mgit 960 m960 system is.
The present study attempted to measure the efficacy of the bactec mgit 960 method for the detection of mycobacteria in extrapulmonary specimens 10, 11. Bd bactec tm mgi tm system software, catalog 445922, lot v5. Bd bactec mgit 960 system is the only high throughput fully automated system to meet the requirement of a tb lab. Contamination rates were practically the same at the three centers both for the bactec 460 system and for the bactec mgit 960 system, while that for lowensteinjensen medium was higher at one of the centers 22. Bd bbl mgit mycobacteria growth indicator tube 7 ml with bd bactec mgit 960 supplement kit. The bactec microbacteria growth indicator tube mgit 960 system was evaluated to determine how it compares with the bactec 460 radiometric system and solid media for recovery of mycobacterium bovis from tissue samples. The complete medium, with oadc enrichment and panta antibiotic mixture, is one of the most commonly used liquid media for the cultivation of mycobacteria. The bactec mgit 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Mean times to detection of mycobacterium tuberculosis n 96 were significantly shorter with mgit 960 12. A total of 506 bovine lymph node samples were collected from abattoirs in the united states and mexico between november 2003 and september 2004. Learn about bd bactec mgit mycobacterial growth indicator tubes. The mgit 960 system was used for primary isolation and standard susceptibility testing of firstline drugs as recommended by the manufacturer. Bd is a leading medical technology company that advances human health in every corner of the world by improving medical research, diagnostics and the delivery of care.
The basic configuration of the software provides all the. Combined with conventional solid medium, it also increases the overall recovery of mycobacteria in culture. Failure of bactec mgit 960 to detect mycobacterium tuberculosis complex within a 42day incubation period. Bactec mycobacterium growth indicator tube mgit 960 system. The system uses an oxygenquenching fluorescent sensor in conjunction with software algorithms. Failure of bactec mgit 960 to detect mycobacterium. Epidemiology of antituberculosis drug resistance the global project on antituberculosis drug resistance surveillance. This system has been extensively evaluated, but very few studies have been carried solely on. Procedure compatibility bd bactec mgit tubes fit into standard procedures for decontamination naclnaoh and waste disposal. Nonradiometric, fully automated system annual capacity of 8,300 tubes for the rapid detection of mycobacteria in clinical specimens, other than blood, as well as antimicrobial susceptibility testing of antituberculosis drugs known as s.
Press the test green leds soft key again to extinguish the green leds. System can accomodate 960 samples at time and thanks towards automatic sensitivity reports generation capability which leads to minimum user interaction. All of the red leds at all the stations should light. In being as efficient as the bactec 460tb system, the new nonradio. The bactec mgit 960 system can expedite the recovery of mycobacteria in culture. A number of studies have reported that the bactec mgit 960 system is efficient, rapid, reliable, safe and fully automated, with the added advantage of continuous monitoring of the culture tubes. In contrast to the bactec 460 instrumentation, the mgit 960 platform is a fully.
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